PRODUCT

E. coli High-Yield Cell-Free Protein Expression Kit

Gene Editing With High Precision

Built for diverse needs and high‑performance workflows, this cell-free protein expression kit leverages the Continuous Exchange Cell-Free (CECF) principle along with an enhanced E.coli lysate to achieve medium-to-preparative-scale protein yields from plasmid DNA. It supports efficient and scalable production of up to 6-mg protein per run.

  • High-yield CECF system enables optimized plasmid-based expression scalable from 0.5 mg to 6 mg per 1-mL reaction
  • Ideal for structural biology workflows including NMR spectroscopy and X-ray crystallography thanks to a preparative-scale yield
  • Flexible amino acid supply allows substitution of methionine for labeling studies
  • Optimized for thermomixer-based operation, ensuring consistent CECF exchange and robust yields

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E. coli High-Yield Cell-Free Protein Expression Kit (5 Reactions)

#CF31ECXPRO-Med
Description

Product Number CF31ECXPRO
Delivery Format Tubes
Source E. coli
Shipping Conditions Dry Ice
Storage Temperature -20 C
Volume 1 mL
Stability 24 months from production; refer to expiration dates for details
Function Continuous-exchange cell-free (CECF) transcription and translation; Plasmid-based protein expression; Expression of toxic or difficult proteins
For Use In Medium-to-preparative-scale protein production workflows; Structural biology; Isotopic labeling for proteins
Intended Use This product is intended for research use only