In IVT, accumulating pyrophosphate (PPi) inhibits T7 and other RNA polymerases. Inorganic pyrophosphatase hydrolyzes PPi to phosphate, preventing product inhibition and sustaining transcription for higher, cleaner mRNA yields at standard reaction conditions.
T4 RNA Ligase II ligates 3′‑OH RNA to 5′‑phosphorylated oligos in a duplex context, suppressing adapter‑dimers and artifacts. It enables cleaner small RNA library preparation and supports dsRNA nick sealing and RNA circularization across scales.
RNase R is a 3′→5′ exoribonuclease that degrades linear RNAs while sparing circular and structured species. Enrich specialized RNA populations and sharpen transcriptome analyses without losing circRNAs or lariat structures.
T7 RNA Polymerase is an enzyme that enables the transcription of RNA from DNA templates. It is an essential tool for various in vitro RNA synthesis applications, offering exceptional specificity and fidelity. This engineered enzyme is manufactured for high purity and is free from contaminating nucleases and DNA, ensuring reliable and consistent performance for your research.
Synthego's RNase Inhibitor is a recombinant protein that safeguards your RNA's integrity by neutralizing RNase activity. It is a crucial reagent for a wide range of molecular biology applications, including in vitro transcription, RNA sequencing, and cDNA synthesis. Ensure the reliability and quality of your experimental results with this high-performance enzyme.