Hot-Start DNA Polymerase, High Fidelity
Proofreading hot‑start polymerase delivering higher fidelity than Taq with robust amplification of mid‑length targets. Produces blunt‑end products for cloning and is well‑suited for NGS library amplification.
- 50x higher fidelity than Taq with proofreading
- Robust amplification of up to 5–10 kb targets
- Produces blunt ends ideal for blunt cloning
- Reliable for NGS library amplification
Taq DNA Polymerase Antibody
Monoclonal antibody reversibly inhibits Taq at ambient temperature and releases during initial denaturation. Reduces primer‑dimers and mis‑priming for cleaner, more specific amplification setups.
- Thermolabile block enables hot‑start PCR
- Reduces primer‑dimers and mis‑priming
- Room‑temperature setup convenience
- Compatible with standard PCR protocols
Standard 5′→3′ DNA polymerase with low 5′→3′ exonuclease and no proofreading. Adds 3′‑A overhangs for TA cloning. Reliable amplification across typical templates and buffers for everyday PCR and genotyping.
- Adds 3′‑A overhangs for TA cloning
- Reliable across common buffers and templates
- Ideal for routine PCR and genotyping
- Low 5′→3′ exonuclease; no proofreading
Equimolar A/C/G/T at 25 mM each simplifies setup and improves run‑to‑run consistency. Ideal for routine and high‑throughput PCR, library construction, and automated pipelines requiring reproducibility.
- Equimolar A/C/G/T at 25 mM each
- Simplifies setup and reduces variability
- Great for routine and HT PCR
- Automation‑friendly performance
High‑Purity dUTP suitable for incorporation by many DNA polymerases. Pair with UNG/UDG to minimize carryover contamination in PCR/qPCR. Also useful for labeling and nick translation workflows.
- Supports UNG/UDG carryover prevention
- Polymerase‑compatible incorporation of dU
- Useful for labeling and nick translation
- Balances performance and contamination control
High Sensitivity Reverse Transcriptase
High‑sensitivity reverse transcriptase designed for highly specific, low‑copy detection and structured RNAs. Excels in standard RT, RT‑qPCR, RNA analysis by primer extension, and RACE with clean backgrounds and consistent yields.
- Sensitive RT for low‑copy targets
- Handles structured RNAs efficiently
- Supports RT‑qPCR, RACE, and primer extension
- Consistent yields with clean backgrounds
HPLC‑purified nucleotides supplied individually at 100 mM for A/C/G/T. Mix and match for optimal performance and balanced incorporation in PCR, qPCR, RT‑PCR, and cloning workflows across instruments.
- ≥99% Purity by HPLC for each nucleotide
- Supplied individually at 100 mM (A/C/G/T)
- Flexible formulation for diverse workflows
- Compatible with PCR, qPCR, RT‑PCR, cloning
Hot-Start Taq DNA Polymerase
Antibody-based Hot-Start Taq DNA Polymerase ensures exceptional specificity and yield in demanding PCR applications. Inactive during setup and rapidly activated by heat, it minimizes primer-dimer formation and nonspecific amplification, enabling clean, reproducible results across diverse templates up to 5 kb.
- Antibody-mediated Hot-Start for fast, precise activation
- High specificity and sensitivity with minimal background
- Robust amplification of low-copy or GC-rich templates
- Ready-to-use format with optimized reaction buffer and MgCl₂
Engineered M-MuLV Reverse Transcriptase
Engineered M‑MuLV RT with reduced RNase H activity and increased thermostability supports reverse transcription up to 55 °C. Capable of high-yield, full-length cDNA synthesis and suitable for RNA‑seq library prep workflows with reliable performance on structurally complex RNA.
- Reduced RNase H with greater thermostability
- RT up to 55 °C for structured templates
- Generates long cDNAs for downstream PCR
- Ideal for RNA‑seq library preparation