This positive control is a validated gRNA sequence targeting RELA with editing efficiency of 80% - 90% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.
This gRNA generates a non-edited control for analyzing SpCas9-edited cells.
Includes 1 nmol of modified sgRNA.
Negative control gRNAs are designed to not target any genomic location in the human & mouse genome.
This gRNA generates a non-edited control for analyzing SpCas9-edited cells.
Includes 1 nmol of modified sgRNA.
This positive control is a validated gRNA sequence targeting Rosa26 in mouse cell lines. Use this control to optimize transfection conditions for maximum editing efficiency with SpCas9.
Includes 1 nmol of modified sgRNA.
This multi-guide positive control is a validated trio of gRNAs targeting TRAC in human cell types.
For the full kit of the positive control with the addition of sequencing and PCR primers as well as Cas9 nuclease, purchase the Transfection Optimization Kit.
Includes 1.5 nmol of modified multi-guide sgRNA.
This eSpOT-ON optimization kit contains:
eSpOT-ON Nuclease, Recombinant Protein - 100 μg
eSpOT-ON Nuclease, mRNA - 100 μg
Positive Control eSpOT-ON gRNA (mod), Human B2M1 - 1.5 nmol
Positive Control eSpOT-ON gRNA (mod), Human TRAC - 1.5 nmol
Negative Control eSpOT-ON gRNA (mod) - 1.5 nmol
Nuclease Free Water
TE Buffer
This SpCas9 optimization kit contains:
SpCas9 Nuclease - Recombinant Protein - 300 pmol
2x Positive Control sgRNA (mod), Human RELA & CDC42BPB - 1 nmol each
Negative Control sgRNA (mod) #1 - 1 nmol
PCR Primer Mix (RELA & CDC42BPB) - 20 µl each
4x Sequencing Primers (RELA & CDC42BPB) - 20 µl each
Nuclease-free Water & TE Buffer - 1.5 ml each
10 mM Tris, 1 mM EDTA, pH 8.0
Includes 5 tubes, 1.5ml each
Includes 5 tubes, 1.5ml each