This kit is designed for users to optimize their CRISPR transfection protocols and become familiar with Synthego’s chemically modified sgRNAs and CRISPR-Cas9 technology. The manual includes a detailed stepwise protocol to guide users right from designing primers to analyzing editing efficiencies.

Synthego’s CRISPR Controls Kit (Human) is an easy-to-use kit-based approach to CRISPR-Cas9 that enables consistent, robust, and reproducible genome editing in a variety of human cell types. The kit contains two chemically modified positive control sgRNAs and one chemically modified non-targeting (negative) control sgRNA.

Including positive and negative controls is vital to understanding the effectiveness of your CRISPR-Cas9 experiments and to guide experimental changes that are specific to your gene. Researchers can use the Controls Kit (Human) before or alongside their CRISPR experiments to evaluate their technical and experimental design.

This Controls Kit (Human) manual includes all the following essential details, complete with a troubleshooting guide at the end:

  • Preparation for editing: design, order, and test primers for genomic PCR
  • sgRNA handling and storage instructions
  • Ribonucleoprotein (RNP) complex formation
  • Nucleofection
  • Extraction of genomic DNA
  • ICE analysis for editing efficiency

Download your Controls Kit (Human) manual today!