Interested in doing CRISPR knock-ins but are not sure where to start? Here are some recommendations and advice on how to design guide RNA and a DNA donor template for homology-directed repair. We also provide information on how to optimize a knock-in experiment to get the best results.

Synthego has created a Tips and Tricks series to provide useful information on how to enhance experimental techniques and protocols for CRISPR applications. Building your CRISPR skill set will enable you to take your genome editing experiments to the next level.

The insertion (“knock-in”) of an exogenous genetic sequence into a genome can be used in a wide variety of applications. Yet, the efficiency of knock-ins is notoriously low relative to that of knockouts. We’ve compiled some tips and tricks on how to increase your chance of a successful outcome.

We cover the following areas:

  • Guide RNA design
  • Donor DNA template design
  • Optimization of experimental conditions
  • Conducting a knock-in experiment

The knock-in of genetic sequences hold immense promise in biological research. Join your colleagues in exploring what you can discover with this powerful technique. Don’t be intimidated to take on the challenge of knock-ins, this Tips and Tricks guide will help lead you to success!