Protein format

This User Guide offers key details on storing, handling, and initiating experiments with our eSpOT-ON nuclease protein and gRNA.

Synthego’s eSpOT-ON nuclease is an engineered high-fidelity Cas9 variant optimized for therapeutic applications. Delivered in protein format, eSpOT-ON offers high on-target editing efficiency, low off-target activity, and reduced risk of chromosomal translocations, making it a safer choice for precise cell and gene therapy workflows.

This User Guide is specifically tailored for working with the eSpOT-ON nuclease protein and gRNA. Learn more about:

  • Best practices for storing eSpOT-ON protein and gRNA
  • Instructions on hydrating gRNA
  • Recommended gRNA controls for eSpOT-ON nuclease protein
  • Tips to optimize your transfection setup
  • Guidance on how to analyze CRISPR edits with eSpOT-ON protein

Download the eSpOT-ON nuclease protein and gRNA User Guide to get started developing the next life saving cell and gene therapy with eSpOT-ON!