Gene knockouts, in which genes are inactivated so that they no longer make functional protein, are used to address a variety of scientific questions. The CRISPR gene editing tool has made knocking out genes easier than ever before.

In this eBook, we walk through the step-by-step process of knocking out a gene in cell culture using CRISPR to produce a knockout cell pool. We summarize the direct applications of knockout cell pools and describe how they are used to make clonal cell lines.

Our eBook includes the following areas:

  • Introduction to CRISPR
  • Workflow of a Knockout Experiment
  • Details on Synthego’s Knockout Cell Pools
  • Applications of the Knockout Technique