Our CRISPR lipofection protocol was designed to allow highly efficient transfection of RNP complexes into standard cell lines without using any specialized equipment. This protocol is best optimized for medium-throughput experiments in 24-well plates.

This CRISPR lipofection protocol describes how to deliver ribonucleoprotein (RNP) complexes that consist of purified Cas9 nuclease duplexed with chemically modified synthetic single guide RNA (sgRNA) into standard immortalized cells. Using our transfection protocol, you will learn how to utilize CRISPR transfection techniques that ensure optimal delivery of CRISPR components to your cells, resulting in high-efficiency genome engineering.

When proper CRISPR transfection techniques are combined with high-quality synthetic guide RNA, you'll be able to achieve unparalleled consistency between your experimental replicates.

Download your Immortalized Cell Lipofection Protocol copy today!