PRODUCT

T7 RNA Polymerase

High-yield, high-purity RNA synthesis.

T7 RNA Polymerase is an enzyme that enables the transcription of RNA from DNA templates. It is an essential tool for various in vitro RNA synthesis applications, offering exceptional specificity and fidelity. This engineered enzyme is manufactured for high purity and is free from contaminating nucleases and DNA, ensuring reliable and consistent performance for your research.

Synthesizes RNA with high yield and purity for reliable results.
Shows high specificity for T7 promoters, ensuring precise transcription.
Supports diverse applications, including mRNA and CRISPR guide RNA synthesis.

Select a Size

T7 RNA Polymerase (5000 Units)

#R25T7POL-SM

Product Specifications Description Quality Resources

Contents

Recombinant, engineered T7 RNA Polymerase (50 U/μl) in storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.9

Storage temperature is -20±10°C

T7 RNA Polymerase Reaction Buffer at 10X concentration formulation: 40 mM Tris-HCl (pH 7.9 at 25°C), 6 mM MgCl2, 2 mM spermidine, 10 mM NaCl

Need a custom formulation? Contact us for availability.

Unit Definition

One unit of T7 RNA Polymerase is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C.

Quality

A sample from each lot of T7 RNA Polymerase is tested in the following assays:

Functional Assay (in vitro transcription)
E. coli DNA Contamination assay
RNase Contamination assay
Protein Purity (≥ 90%)

Product Description

T7 RNA Polymerase is a widely used enzyme that facilitates RNA transcription by synthesizing RNA from DNA templates controlled by T7 phage promoters. The enzyme exclusively recognizes T7 promoters, ensuring efficient and controlled RNA production without interference from off-target sequences.

Our T7 RNA polymerase sets a new standard for performance and efficiency. A recent publication demonstrated its superior capabilities, showcasing its high reaction rate and reduced dsRNA by-products, ensuring exceptional RNA purity even in small-scale reactions. When scaled up, it maintained high RNA purity and yield, making it highly effective across various sensitive RNA applications. Most notably, our enzyme achieved the highest RNA purity and yield in just half the incubation time, making it the clear choice for research and RNA therapeutic development.

Key Features

Superior Performance: Our T7 RNA Polymerase gives you high RNA purity and yield in half the incubation time compared to other recombinant T7 polymerases.
High and Reliable Quality: ISO 13485 manufacturing processes and quality control measures verify high purity and absence of contaminating nucleases or DNA, ensuring the enzyme’s consistent performance and reliability for sensitive research applications.
High-Fidelity RNA Production: Its specificity for T7 promoters ensures precise transcription, reducing transcription errors and unwanted RNA products.
Broad Utility: Supports diverse applications such as mRNA synthesis, labeled RNA probe production, CRISPR guide RNA transcription, and RNA structural studies.

Key Applications

In Vitro RNA Transcription: Synthesizing RNA in vitro, with precise control over RNA production for downstream research and functional studies.

RNA Probe Labeling: Creating labeled RNA probes to detect specific nucleic acid sequences in hybridization assays for molecular biology and diagnostics.

mRNA Synthesis: Supports efficient mRNA synthesis with high yield and fidelity for use in research and therapeutic applications including vaccine development, cellular reprogramming, and gene therapy experiments.

CRISPR Guide RNA Production: Transcribing guide RNAs with consistency and accuracy for CRISPR applications, especially pegRNAs, highly customized gRNAs, and longmers.

RNA Structure and Function Studies: Producing RNA for structural analysis, such as studies on RNA folding or RNA-protein interactions.

Synthetic Biology and Aptamer Production: Supporting synthetic biology workflows, including antisense RNA production, RNA aptamer creation, and other applications requiring precise RNA transcription.

Quality Control Assays for T7 RNA Polymerase

The manufacturing of T7 RNA Polymerase includes rigorous quality control testing to ensure the enzyme meets the highest standards of purity, activity, and performance. Each batch is carefully evaluated using the following assays:

Functional Assay (in vitro transcription)

This assay tests the enzyme's ability to transcribe RNA from a DNA template under controlled in vitro conditions. By mimicking a standard RNA transcription reaction, this test assesses both the activity and efficiency of the T7 RNA Polymerase. The reaction includes a DNA template containing a T7 promoter, ribonucleotides (NTPs), and reaction buffer components like magnesium. After incubation, RNA output is quantified using spectrophotometric measurements or gel electrophoresis to evaluate yield and size. Activity is assessed based on RNA production levels and transcript integrity. Successful results indicate the enzyme can reliably perform its primary function, producing high-quality RNA suitable for downstream applications, such as RNA probe synthesis, mRNA production, and CRISPR guide RNA transcription.

E. coli DNA Contamination Assay

Manufactured via recombinant expression in Escherichia coli, T7 RNA Polymerase is tested for residual genomic DNA from the host organism. This assay uses sensitive detection methods like quantitative PCR (qPCR) or digital PCR using primers specific to E. coli genomic sequences to confirm the absence of E. coli DNA contamination, which could otherwise introduce unintended sequences into RNA transcripts. These techniques can detect even trace amounts of host DNA. A negative result confirms that the T7 RNA Polymerase preparation is free from E. coli contaminants and suitable for sensitive research and therapeutic applications.

RNase Contamination Assay

This assay determines whether RNase, an enzyme that degrades RNA, is present in the preparation. RNase contamination can significantly compromise RNA integrity, leading to degraded or incomplete RNA transcripts. The test involves incubating a known RNA sample with the T7 RNA Polymerase preparation under physiological conditions. RNA degradation is evaluated using gel electrophoresis or fluorescence-labeled RNA decay assays. A negative result confirms the enzyme is RNase-free, safeguarding the integrity of RNA products for downstream applications like RNA structural analysis and gene therapy workflows.

Protein Purity (≥ 90%)

The protein purity test evaluates the proportion of T7 RNA Polymerase present in the final preparation, ensuring it constitutes at least 90% of the total protein content. High protein purity reduces the likelihood of contaminants interfering with the enzyme's performance. Protein purity is evaluated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or high-performance liquid chromatography (HPLC). These methods quantitatively determine the proportion of T7 RNA Polymerase relative to total protein content in the preparation. A purity of ≥ 90% ensures minimal contamination, reliable enzyme activity, and compatibility with demanding applications such as mRNA synthesis and CRISPR guide RNA production.

These comprehensive quality control assays provide confidence in the reliability and effectiveness of T7 RNA Polymerase. By verifying functionality, purity, and the absence of contaminating activities, these tests ensure the enzyme consistently delivers the precision and integrity required for RNA transcription in both research and therapeutic contexts.

RESOURCES

Download the User Guide for Storage and Handling Recommendations

T7 RNA Polymerase User Guide