Guide RNA design is crucial for CRISPR Experiments because it can directly determine the specificity and efficiency of the editing action, which is essential for accurate genome editing.
To design your own guide RNA, select your target sequences using your favorite CRISPR design platform, such as CHOPCHOP, Benchling, CRISPOR, or Cas-Designer CRISPR RGEN Tools.
PAM sequences for eSpOT-ON, hfCas12Max, and SpCas9 are below:
To order your guide RNA, upload your target sequences without the PAM sequence into our easy-to-use ordering interface so we can synthesize your guide RNA. For experiment result analysis via sanger sequencing, we recommend the ICE tool hosted by Editco.
BE-Designer, Benchling, BE-Hive, and SpliceR are all tools that support guide design for base editing, including both ABE and CBE editing options. Synthego now offers the Accubase CBE for customers, which has a canonical PAM 5' - NGG - 3'.
For results analysis, EditR provides Sanger Sequencing analysis, and CRISPResso2 for NGS analysis.
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