PRODUCT

T7 RNA Polymerase

High-yield, high-purity RNA synthesis.

T7 RNA Polymerase is an enzyme that enables the transcription of RNA from DNA templates. It is an essential tool for various in vitro RNA synthesis applications, offering exceptional specificity and fidelity. This engineered enzyme is manufactured for high purity and is free from contaminating nucleases and DNA, ensuring reliable and consistent performance for your research.

Synthesizes RNA with high yield and purity for reliable results.
Shows high specificity for T7 promoters, ensuring precise transcription.
Supports diverse applications, including mRNA and CRISPR guide RNA synthesis.

Select a Size

T7 RNA Polymerase (5000 Units)

#R25T7POL-SM

Product Overview Quality Resources

Overview

Do you rely on a steady supply of RNA enzymes for continuous RNA transcription?

We offer superior enzyme performance, volume-based pricing, and reliable delivery schedules to support your essential applications without interruptions.

Contents

Recombinant, engineered T7 RNA Polymerase (50 U/μl) in storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.9

Storage temperature is -20±10°C

T7 RNA Polymerase Reaction Buffer at 10X concentration formulation: 40 mM Tris-HCl (pH 7.9 at 25°C), 6 mM MgCl2, 2 mM spermidine, 10 mM NaCl

Need a custom formulation? Contact us for availability.

Unit Definition

One unit of T7 RNA Polymerase is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C.

Quality

A sample from each lot of T7 RNA Polymerase is tested in the following assays:

Functional Assay (in vitro transcription)
E. coli DNA Contamination assay
RNase Contamination assay
Protein Purity (≥ 90%)

Product Advantages

Superior Performance: Our T7 RNA Polymerase gives you high RNA purity and yield in half the incubation time compared to other recombinant T7 polymerases.
High and Reliable Quality: ISO 13485 manufacturing processes and quality control measures verify high purity and absence of contaminating nucleases or DNA, ensuring the enzyme’s consistent performance and reliability for sensitive research applications.
High-Fidelity RNA Production: Its specificity for T7 promoters ensures precise transcription, reducing transcription errors and unwanted RNA products.
Broad Utility: Supports diverse applications such as mRNA synthesis, labeled RNA probe production, CRISPR guide RNA transcription, and RNA structural studies.

Product Overview

T7 RNA Polymerase is a widely used enzyme that facilitates RNA transcription by synthesizing RNA from DNA templates controlled by T7 phage promoters. With a molecular weight of approximately 99 kDa, it is a vital tool for in vitro RNA synthesis, offering unmatched specificity and fidelity. The enzyme exclusively recognizes T7 promoters, ensuring efficient and controlled RNA production without interference from off-target sequences.

Our T7 RNA polymerase sets a new standard for performance and efficiency. A recent publication demonstrated its superior capabilities, showcasing its high reaction rate and reduced dsRNA by-products, ensuring exceptional RNA purity even in small-scale reactions. When scaled up, it maintained high RNA purity and yield, making it highly effective across various sensitive RNA applications. Most notably, our enzyme achieved the highest RNA purity and yield in just half the incubation time, making it the clear choice for research and RNA therapeutic development.

Our T7 RNA Polymerase is manufactured through recombinant expression in Escherichia coli, under ISO 13485 conditions and stringent quality control measures, verifying high purity and activity levels. Each batch is tested to confirm the absence of DNA and RNase contamination, preserving RNA integrity and minimizing experimental variability. These attributes make it ideal for high-fidelity RNA production across a wide range of applications in both research and therapeutic development.

To further safeguard RNA integrity during in vitro transcription and downstream processes, combining T7 RNA Polymerase with an RNase inhibitor enzyme is highly recommended. RNase inhibitors play a complementary role by neutralizing any residual RNase activity, preventing RNA degradation and enhancing reliability across sensitive workflows. This synergy ensures optimal performance in applications such as RNA sequencing, cDNA synthesis, and mRNA-based therapeutic development. For additional support against RNA degradation, explore our RNase inhibitor product page.

Key Applications

In Vitro RNA Transcription: T7 RNA Polymerase is essential for synthesizing RNA in vitro, providing researchers with precise control over RNA production. It is commonly used to produce RNA for downstream research and functional studies.

RNA Probe Labeling: The enzyme is widely utilized for creating labeled RNA probes to detect specific nucleic acid sequences in hybridization assays, supporting molecular diagnostics and research applications.

mRNA Synthesis: T7 RNA Polymerase supports efficient mRNA synthesis for use in research and therapeutic applications. Its ability to produce mRNA with high yield and fidelity makes it an integral tool in vaccine development, cellular reprogramming, and gene therapy experiments.

CRISPR Guide RNA Production: T7 RNA Polymerase is commonly used to transcribe guide RNAs for CRISPR applications, especially pegRNAs, highly customized gRNAs, and longmers. Its precision ensures consistent and accurate transcription of gRNAs for efficient CRISPR-based gene editing.

RNA Structure and Function Studies: Researchers use this enzyme to produce RNA for structural analysis, such as studies on RNA folding or RNA-protein interactions, advancing RNA biology understanding.

Synthetic Biology and Aptamer Production: T7 RNA Polymerase supports synthetic biology workflows, including antisense RNA production, RNA aptamer creation, and other applications requiring precise RNA transcription.

QUALITY

Quality Control Assays for T7 RNA Polymerase

The manufacturing of T7 RNA Polymerase includes rigorous quality control testing to ensure the enzyme meets the highest standards of purity, activity, and performance. Each batch is carefully evaluated using the following assays:

Functional Assay (in vitro transcription)

This assay tests the enzyme's ability to transcribe RNA from a DNA template under controlled in vitro conditions. By mimicking a standard RNA transcription reaction, this test assesses both the activity and efficiency of the T7 RNA Polymerase. The reaction includes a DNA template containing a T7 promoter, ribonucleotides (NTPs), and reaction buffer components like magnesium. After incubation, RNA output is quantified using spectrophotometric measurements or gel electrophoresis to evaluate yield and size. Activity is assessed based on RNA production levels and transcript integrity. Successful results indicate the enzyme can reliably perform its primary function, producing high-quality RNA suitable for downstream applications, such as RNA probe synthesis, mRNA production, and CRISPR guide RNA transcription.

E. coli DNA Contamination Assay

Manufactured via recombinant expression in Escherichia coli, T7 RNA Polymerase is tested for residual genomic DNA from the host organism. This assay uses sensitive detection methods like quantitative PCR (qPCR) or digital PCR using primers specific to E. coli genomic sequences to confirm the absence of E. coli DNA contamination, which could otherwise introduce unintended sequences into RNA transcripts. These techniques can detect even trace amounts of host DNA. A negative result confirms that the T7 RNA Polymerase preparation is free from E. coli contaminants and suitable for sensitive research and therapeutic applications.

RNase Contamination Assay

This assay determines whether RNase, an enzyme that degrades RNA, is present in the preparation. RNase contamination can significantly compromise RNA integrity, leading to degraded or incomplete RNA transcripts. The test involves incubating a known RNA sample with the T7 RNA Polymerase preparation under physiological conditions. RNA degradation is evaluated using gel electrophoresis or fluorescence-labeled RNA decay assays. A negative result confirms the enzyme is RNase-free, safeguarding the integrity of RNA products for downstream applications like RNA structural analysis and gene therapy workflows.

Protein Purity (≥ 90%)

The protein purity test evaluates the proportion of T7 RNA Polymerase present in the final preparation, ensuring it constitutes at least 90% of the total protein content. High protein purity reduces the likelihood of contaminants interfering with the enzyme's performance. Protein purity is evaluated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or high-performance liquid chromatography (HPLC). These methods quantitatively determine the proportion of T7 RNA Polymerase relative to total protein content in the preparation. A purity of ≥ 90% ensures minimal contamination, reliable enzyme activity, and compatibility with demanding applications such as mRNA synthesis and CRISPR guide RNA production.

Significance of Assay Results

These comprehensive quality control assays provide confidence in the reliability and effectiveness of T7 RNA Polymerase. By verifying functionality, purity, and the absence of contaminating activities, these tests ensure the enzyme consistently delivers the precision and integrity required for RNA transcription in both research and therapeutic contexts.

RESOURCES

Download the User Guide for Storage and Handling Recommendations

T7 RNA Polymerase User Guide