Guide RNAs come in two formats: two-piece cr:tracrRNA and single guide RNA (sgRNA), both with their advantages and disadvantages. At Synthego, we understand how important the right guide RNA format is for a successful CRISPR experiment. In this application note, we compare the editing efficiencies of Synthego’s cr:tracrRNA, cr:tracrRNA from other vendors, and Synthego’s sgRNA. Learn which is the best option for your experiment needs.

Choosing the correct guide RNA format is critical to the success of your CRISPR experiment. Deciding which is the right one for you may be guided by a number of factors, including time and price. Importantly, understanding the differences between the available guide RNA options and their associated editing efficiencies will help inform scientists to make the best decision for their CRISPR experiment.

In this application note, we compare editing efficiencies obtained using cr:tracrRNAs from Synthego, those from other vendors, and Synthego’s sgRNAs. We compared editing efficiencies across vendors and guide formats of 13 randomly selected genes (3 targets per gene) and found the following:

  • Out of all the guides tested, Synthego’s sgRNA offers the highest likelihood of a successful edit
  • The two-piece cr:tracr guides from different vendors vary in editing efficiency
  • The two-piece cr:tracr guides from Synthego outperform other cr:tracr guides

Learn more about our competitive analysis and findings to help you decide which guide RNA option is the best for you. Get a free copy of our Application Note now!