mRNA format

This User Guide covers important guidelines for storage, preparation, and getting started with our eSpOT-ON nuclease mRNA and gRNA.

eSpOT-ON is Synthego’s engineered high-fidelity Cas9 variant, designed for therapeutic gene editing. Delivered in mRNA format, eSpOT-ON provides superior on-target activity, low off-target effects, and a reduced risk of chromosomal translocations. Validated in preclinical studies, eSpOT-ON nuclease mRNA and gRNA are ready to support your next breakthrough in cell and gene therapy development.

This User Guide provides step-by-step support for working with eSpOT-ON nuclease mRNA and guide RNA (gRNA), including:

  • Storage recommendations for eSpOT-ON nuclease mRNA and gRNA
  • Instructions for gRNA preparation and handling
  • Recommended gRNA controls for eSpOT-ON nuclease mRNA
  • Key tips for efficient delivery and transfection
  • Guidance on evaluating and interpreting editing outcomes

Download the eSpOT-ON nuclease mRNA and gRNA User Guide to accelerate your next therapeutic innovation.