Gene editing of human cancer cell lines are useful to researchers looking to understand cancer cell biology and genetics. In this protocol, researchers at the University of California, San Francisco, in conjunction with Synthego, describe step by step instructions on how to edit human 143B osteosarcoma cells by CRISPR-Cas9 using electroporation.

Synthego’s chemically modified synthetic sgRNAs are of exceptional purity and consistently drive high editing frequencies, making them a great choice for CRISPR-editing experiments.

This protocol describes how to electroporate human 143B osteosarcoma cells with ribonucleoprotein (RNP) complexes that consist of purified Cas9 nuclease duplexed with chemically modified synthetic guide RNA (sgRNA). Synthego’s chemically modified sgRNAs are designed to resist exonuclease degradation and innate intracellular immune cascades that can lead to cell death.

This protocol provides all the necessary details to carry out your CRISPR experiment including:

  • Required transfection reagents and equipment
  • Detailed electroporation protocols including pre- and post-electroporation steps
  • Data demonstrating CRISPR-mediated editing in 143B cells.

Download the Human 143B Cell Electroporation protocol today!