This User Guide provides essential information regarding storage, handling, quantification, and dilution of our research use sgRNAs, and preparing RNPs in a simple and easy-to-follow guide. It includes instructions for handling in tube and plate formats.

Our research use single guide RNA (sgRNA) are best-in-class synthetic sgRNA that are easy to use and enable you to achieve your highest editing efficiency. With their fast synthesis and delivery, we recommend reviewing this User Guide in advance so you can get started with your CRISPR experiments as soon as your sgRNA arrive.

In this Research sgRNA User Guide, you will learn the following:

  • Rehydrating the sgRNA
  • Quantifying and diluting the sgRNA
  • Links to our recommended transfection protocols to use the research grade sgRNA
  • Analyzing the editing efficiency

Download your Synthetic gRNA User Guide now!